不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系

 不同生长期蛋鸡的体脂水平和肝脏脂肪酸合成酶活性的关系田维熙,董妍,权晖,陈文峰（中国科学院研究生院生物教学部,北京１０００３９）动物体脂的控制是一个复杂过程．不同种类不同年龄的动物体脂水平有很大差异,控制机制是什么,哪些是关键的控制因素？不久前我们曾...     

GPX－abzyme对受XO／HX系统损伤的心肌线粒体的保护作用

探讨了研制的具有谷胱甘肽过氧化物酶活力的含硒抗体酶（ＧＰＸ－ａｂｚｙｍｅ）对于受损心肌线粒体的保护作用,利用牛的心肌线粒体为实验材料,通过线粒体的膨胀度、脂质过氧化物含量、ＣＣＯ活力变化及电镜观察等几个方面证明ＧＰＸ－ａｂｚｙｍｅ能抵抗ＸＯ／ＨＸ系统产生的自由基的损伤作用,ＥＳＲ研究也表明ＧＰＸ－ａｂｚｙｍｅ能明显降低ＸＯ／ＨＸ损伤系统中的自由基含量。     

木瓜蛋白酶PPAⅢ自水解作用

木瓜蛋白酶ＰＰＡⅢ自水解作用王秀艳,周慧,葛玉斌,李惟（吉林大学分子生物学系,长春１３００２３）ＰＰＡⅢ、ｐａｐａｉｎ、ｐｅｐｓｉｎｅ、ｓｔｅｍｂｒｏｍｅｌａｉｎ等都属于以Ｃｙｓ－ＳＨ为活性中心的酶,同种分子之间存在着一种相互切割的趋势,称为酶自水解...     

A NEW SPECIES OF GENUS GRYPOCENTRUS RUTHE (HYMENOPTERA: ICHNEUMONIDAE)

Abstract  One new species of Grypocentrus , namely G. kasparyani , collected in Shenyang, China, is described.     

Laminin and Neuropeptide Y Are Increased by Synapsin Transfection in Cultured NG108-15 Neuroblastoma/Glioma Hybrid Cells

Abstract: We have investigated the presence and expression of laminin and neuropeptide Y (NPY) in several NG108-15 cell lines transfected with synapsin Ib, IIa, or IIb. The content of laminin, a basal membrane glycoprotein that promotes adhesion and induces neurite outgrowth and neuronal differentiation, was increased in all transfected cell lines examined. In cells that were chemically differentiated with prostaglandin E₁ plus 3-isobutyl-1-methylxanthine, laminin levels were increased even further. The content of NPY, suggested to be a neurotransmitter/neuromodulator in peripheral sympathetic neurons as well as in central neurons, was also increased in all transfected cell lines examined. Immunohistochemical analysis combined with confocal laser microscopy showed that NPY staining was granular and very often enriched in neuritic varicosities. The distribution and the staining pattern of NPY were consistent with storage of NPY in large dense-cored vesicles. The results indicate that, in differentiated neurons, the synapsins increase the levels of a neuropeptide transmitter stored in large dense-cored vesicles and of an extracellular matrix protein associated with neuronal maturation.     

Human immunodeficiency virus type 1 envelope protein does not stimulate either prostaglandin formation or the expression of prostaglandin H synthase in THP-1 human monocytes/macrophages.

Prostaglandin E2 is observed at elevated levels during human immunodeficiency virus (HIV) infection and thus may contribute to the HIV-dependent immunosuppression. The mechanisms responsible for this increase are not understood. Evidence indicates that the viral envelope proteins perturb membrane signaling mediated by the CD4 receptor, suggesting that the free envelope protein and/or the intact virus may be responsible for the increase in prostaglandin E2 levels. In this study, we have used THP-1 human monocytes and THP-1 cells differentiated by 12-O-tetradecanoylphorbol-13-acetate treatment into macrophages to determine if the HIV envelope protein, gp120, or an anti-CD4 receptor antibody stimulates prostaglandin formation by interacting with the CD4 receptor. Incubation of THP-1 cells with OKT4A antibody greatly stimulated the CD4-p56lck receptor complex as estimated by enhanced p56lck autophosphorylation, while the gp120 gave small but significant responses. Monocytic THP-1 cells poorly metabolized arachidonic acid to prostaglandin E2 and thromboxane B2 as measured by high-pressure liquid chromatography analysis. Western blot (immunoblot) and Northern (RNA) blot analyses revealed that unstimulated monocytes expressed little prostaglandin H synthase 1 and 2 (PGHS-1 and -2). Incubation of the monocytes with lipopolysaccharide, OKT4A, or gp120 did not increase the formation of prostaglandins. The expression of PGHS-1 or PGHS-2 was also not increased. Differentiation of the monocytes to macrophages by 12-O-tetradecanoylphorbol-13-acetate treatment resulted in increased expression of PGHS-1 and increased formation of prostaglandins compared with that for the monocytes. Lipopolysaccharide stimulation of the macrophages increased the formation of prostaglandins and increased the expression of PGHS-2 in the macrophages. However, OKT4A or gp120 preparation, at concentrations that stimulated p56lck autophosphorylation, did not enhance the formation of prostaglandins or the expression of PGHS-1 or PGHS-2. OKT4A and gp120 also did not stimulate the release of arachidonic acid, indicating that phospholipase A2 was not activated by the CD4 receptor in either the THP-1 monocytes or macrophages. These results indicate that activation of the CD4-p56lck receptor signal transduction pathway by the HIV envelope protein does not increase prostaglandin formation.     

康宁木霉CP88329纤维素酶产生条件的研究

从生霉棉布上分离出的康宁木霉ＣＰ８８１０５经诱变处理,获得一株高产纤维素酶的突变株ＣＰ８８３２９。在固体培养基上,２８℃培养７２小时,所产纤维素酶固体曲,以羧甲基纤维素钠为底物酶活力为４８８０ｕ／ｇ;以脱脂棉为底物酶活力为４８０ｕ／ｇ。产酶活力水平均为出发菌的３倍。酶在脱脂棉上作用最适条件为ｐＨ４．５－５．０,４５－５０℃;４５℃保温４ｈ,ｐＨ稳定范围为３．５－６．５;６０℃保温１ｈ,酶活力剩余２０％。酶可用于苎麻布抛光处理和牛仔服“石磨”处理。     

Thermotolerance induced by heat shock in Chlorella

Thermotolerance in cultures of Chlorella zofingiensis was induced by heat shock treatment at supraoptimal temperatures (40and 45 °C for 30 min). Thermotolerance was assayed by two methods: the survival of the cells at 70 °C and the growth of diluted cultures at 35 and 45 °C. A culture without heat shock treatment was unable to grow at 45 °C. According to eletrophoretic analyses, the synthesis of proteins of 95, 73, 60, 43 and 27 kDa was induced by heat shock treatment. The large molecular weight proteins (95, 73, 60 and43 kDa) were present in non-heat treated cells, but the heat shock treatment increased their quantity in cells. The synthesis of a low molecular weight protein (27 kDa) was induced by heat shock treatment. The induced thermotolerance could be inhibited by the presence of an 80S ribosomal translation inhibitor, cycloheximide(CHI). The first 12 amino acid residues from the N-terminus of the27 kDa heat shock induced protein are Val-Glu-Trp-Try-Gly-Pro-Asn-Arg-Ala-Lys-Phe-Leu. This revised version was published online in August 2006 with corrections to the Cover Date.     

异色瓢虫若虫的限域搜索行为

在Ｎａｋａｍｕｔａ装置中,通过对两种我照照条件下（强光照３６８０－４６２０ｌｘ,弱光照１３０－２２０ｌｘ）异色标虫若虫搜索麦蚜行为轨迹的研究,得出摄食麦蚜前后０－１５ｓ搜索行为轨这弯曲角度总和之间差异均显著,摄食后大于摄前;摄食麦蚜前后０－１５ｓ、１５－３０ｓ、３０－４５ｓ、４５－６０ｓ内的各对应停点间距离差异均显著,摄食前大于摄食后;其搜索速度,摄食前显著大于摄食后。     

新型表达载体 pEC34 试用于人 γ 干扰素基因的高表达

构建了一个新的双顺反子表达载体ｐＥＣ３４,它的第一个顺反子是已高表达的ｅｒａ基因的部分序列,并带有原核翻译增强子。当检测ｐＥＣ３４的可用性时,获得了人γ－干扰素的极高表达。目的蛋白占菌体总蛋白的８５．５％,诱导后经ＳＤＳ－ＰＡＧＥ分析,出现分子量为１５．５ｋＤａ左右的蛋白表达带。Ｗｅｓｔｅｒｎ ｂｌｏｔ结果证实为人γ－干扰素。无压力传代３０次表达质粒仍然稳定,表明ｐＥＣ３４可能用作真核蛋白质高表达